Proton T1 Experiments

The procedure for performing a H-NMR T1 measurement (from the online UC Davis manual)

  • log out of the ICON NMR program
  • acquire and process a reference 1D spectrum of the sample
Acquiring T1 Data
  • create a new dataset by typing new into the command line
  • load the t1ir pulse sequence by typing rpar and selecting PROTONT1
  • type edlist into the command line in order to set up a vd list of relaxation delays (it is best to if the number of delays is a number that corresponds to 2x, 8 or 16 are the best). Choose the filename and save the file.
  • In AcquPars set the number of TD points in F1 to the number of vd values
  • In ProcPars set the number of SI points in F1 to the number of vd values
  • In AcquPars set vdlist ot the name of the file with your vd values (or edit the vdlist)
  • In AcquPars make sure all the other parameters (SW, P1, PL1, RG, etc.) match what was used to acquire the reference 1D spectrum.
  • Type zg or click Start Acquisition and collect data.
Processing T1 Data
  • Type rser # where # is a file witha long relaxation delay (i.e., a large value of vd)
  • efp - perform a fourier transform of the single spectrum
  • ph - open the phase correction window and correct the phase of the spectrum
    • you may want to use "Manual Phasing" or "Automatic Phasing" or "Automatic Phasing using PHC0/1"
    • After the executing the phase correction, note the phase correction parameters
    • In ProcPars of the 2D display, enter the phase correction parameters for PHC0 and PHC1 from the last step in the "Phase Correction" section 
  • Return to the 2D display
  • Type xf2
  • Type abs2
  • Click Multiple Display - a new row of buttons appears atop the spectrum window.
    • Select Scan Rows and use the + and - buttons to view the spectra with different vd values
    • Click Return to exit Multiple Display
  • Use the pull-down Analysis > T1/T2 Relaxation. A new window opens that will lead you through the analysis. The fitting function type should be uxnmrt1